Hey Everyone,
First proper day in the lab today! Me and Jo made up 1L of X50 TAE (a buffer which we will be using ALOT of during our project!) and 1L of EET (which is used when making plugs, we will be producing during the project, more stable), we then autoclaved both. Mike also gave us a reminder of our old friends; Moles and Molar Mass!
After the TAE was autoclaved we used it to run our first gel, in order to practise our technique. We diluted our TAE 50 fold and combined it with 8% agarose to make our gel. We pipetted out: 2 microlitres of sample, 2 microlitres of loading buffer and 6 TAE. We ran 9 lanes: 2 Lambda Hind III, pure B1 DNA, pure PUC BAM H1, pure PUC ECO R1, pure PBC SK+ ECO R1, pure PBC SK+ BAM H1, raw PUC and raw PBC,at 110V for an hour. We then stained up our gel with ethidium bromide and used the UV spectrometer. Our gel turned out fairly well, although Mike said that the ladders were a little messy, and that could be due to the temperature. There was also a small nick in the gel, which created some dragging.
Excited for tomorrow! 🙂