A rather sleepy blog today waiting around in…

A rather sleepy blog today, waiting around in the lab for things to be ready is very tiring. With Rachael Simpson, we finished making our broth cultures into stabs and slopes and left them to incubate overnight. A slope is a universal jar filled (in this case) with nutrient agar and left to set at an angle, the target bacteria is innoculated onto the surface of the set, sloped agar, giving a larger surface area for the culture to form a bacterial lawn, these can then be stored for couple of months. A stab jar is a bijou jar also filled with nutrient agar, the target culture’s cells are stabbed a coulpe of times into the agar, then incubated overnight. The bijou jar can be stored for years. Both these procedures were carried out under aseptic techniques.