Today Rachael and I started to isolate single…

Today Rachael and I started to isolate single colonies from the agar plates we incubated yeserday and streak them onto fresh nutrient agar plates. These have been placed in the 37C incubator overnight. Hopefully if there is growth, we can start to Gram stain them, further research will help us to correctly identify them and make up more stabs and slopes. We also streaked a number of yeasts onto MEA and placed them into the 25C incubator. I learned that it is better to incubate some cultures at a lower temperature for longer than at a higher temperature as this could possibly kill some cultures, especially if the higher temperature is at the top end of their tolerance range. Whilst looking at the agar plates from yesterday the P.S. aeruginosa looked cloudy and smeary, very similar to B. cereus and not the expectation of P.S. aeruginosa. We Gram stained a couple of slides using P.S. aeruginosa they were Gram positive, our research showed that it should have been Gram negative, rod shaped. Because of this we are going to streak P.S aeruginosa on Pseudimonas CFC selective agar. Hopefully tomorrow we shall see some results. Thanks to Nicola and Mike i am learning to tag,italics and google chrome.