Hey all!
Unfortunately my competent cells I transformed didn’t grow so I spread plated the cells I grew yesterday again to see if it’s my spread plating technique isn’t correct. I then used the competent cells I made yesterday and transformed them again today using the plasmids pALA1029 C, pOG4 C, large pOG04 and pOG.003 and then spread plated them. Both sets of plates are currently growing in a incubator at 37 degree’s overnight. Really hoping they grow so I can do alkaline lysis tomorrow!
Soph 🙂