Hello,
So my Antibiotic – Cefotaxime has arrived! 🙂
I am now proceeding to follow all of the methods that I used with Ampicillin, just with the Cefotaxime instead and changing them slightly. I made up agar with 4mg/ml of cefotaxime in, and filtered 100ml of Brayford Water onto 2 of the plates. This morning I only had 3 colonies on just one of the plates. This concentration of Antibiotic is quite high for E.coli to be resistant too, so in a way the 3 colonies are highly resistant which is good, but I may have to start doing more plate sto get more out or maybe the rainy weather yesterday affected the amount of E.coli present.
I have now placed these colonies into broths containing cefotaxime again, and tomorrow I will proceed to do Alkaline Lysis with these samples. Once I have a few more, I will run them on a gel and see what banding patterns are going on 🙂