Hi,
Started the day Gram staining E.coli a strain i dont have, this is another cultue confirmed so i then made a broth which i am incubating overnight at 37C. Spent most of the day waiting for cells to grow to an OD of 1, though 0.8 – 1.5 would be within range. Nicola went through serial dilution with me and showed me how to do the first set, i then completed the following three, broth culturing -4 dilution and spread plating -5 and -5.5 dilutions onto LB agar plates and incubated them overnight at 37C. Hopefully tomorrow we will have enough growth to count colonies (100 would be nice) then we can do replica plating. Had another Mike Shaw masterclass and debate (loving lab life xx).
Hi Started the day Gram staining E coli…
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Mike Shaw masterclass…? Sounds painful. What were we discussing; the pros and cons of pre and post staining gels with ethidium bromide and SYBR green. We didn’t get into the many alternative stains that are available, and the different methods of pre-staining (in-gel/in-well).