Just a question, Faris and I were considering an alternative to purifying and isolating bacteriophages, do we have access to an Ultracentrifuge which reaches 13000G? We would be able to pellet bacteriophages. I am new so, I do not know about all of our lab equipment.
Just a question Faris and I were considering…
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Do you mean 13,000? Sounds more like something that may need 130,000 to me…. But if it is 13,000 then any benchtop will do it. Which I why I think it’s wrong as these aren’t ultracentrifuges.
Sorry the protocol I read suggested an ultracentrifuge for some reason. Now I believe I found a better one, suggesting that I should set it to 25,000 x g for 2 hours (possible on benchtops). From what I understand this would most likely leave bacterial cell debris and possibly DNA in the pellets. To avoid DNA contamination in PCR, would I have to treat the pellets with DNase/RNase? Or would centrifugation (maybe in PEG?) and micron filtering be enough?