Hi everyone, Faris and i started the day by making up 800ml of nutrient agar and 500ml of ringers. 2hr lunch whilst these autoclaved, 121c for 15minutes, but takes about an hour and half for the pressure to release and the temperature to drop below 80C. After lunch we poured the nutrient plates, then we made broth cultures and inubated them overnight at 37C, except B.cereus which is incubated at 30C. Then Rosie, Faris and I made agarose gels for use tomorrow. Had a great time watching Steve make up TAE buffer and making EDTA and learning how to pH it.
Hi everyone Faris and i started the day…
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