Hey all!
Today I have transformed some competent cells (DH5 alpha E.coli cells) and used four different plasmids pOG04, pOG4, pALA1029 and pOG4101 (pOG 4.003) and spread plated all the plasmids once they had been transformed and I have put them in a incubator overnight at 37 degrees! Tomorrow I am going to do alkaline lysis (plasmid DNA extraction) in the morning and then learn how to use restriction enzymes and do a restriction enzyme digest. I also plan to do gel electrophoresis with the different plasmids. I finally feel I’ve got the knack for using pipettes (A bit late I know) but i feel alot more confident with them.
Hope to see you all tomorrow! 🙂
Hey all Today I have transformed some competent…
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