Hey all!
My plasmids I transformed and re-transformed from the competent cells I made up on monday didn’t grow overnight unfortunately so I’ve had to start from scratch and made up some DH5a e.coli cells which took over 5 hours to grow today! I’m transforming my cells tomorrow and I can then make some broths up so I can finally do alkaline lysis and gel electrophoresis. Nikki’s helped me to identify where i’ve been going wrong so I’m hoping to finally get some results!
Seeya tomorrow.
Soph 🙂
Hey all My plasmids I transformed and re…
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