As i expected, pALA1029, pOG04 DRW and pOG4003 have once again shown no growth, almost in sympathy the serial dilution i performed yesterday yielded poor results. The spread plates only showed colony growths of 1-14, as i was looking for colonies around 100, i decided that it was not worth replica plating. I have still carried out a serial dilution from the overnight broth cultures and spread plated them onto LB agar plates and left them to incubate at 37C overnight. I do not expect any growth based on todays results but one can hope!
As i expected pALA1029 pOG04 DRW and pOG4003…
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