Right so today’s been a pretty good day…

Right so today’s been a pretty good day. My Broths were looking pretty good which was a good start. Managed to make some soft agar in tubes which was pretty cool. My nutrient agar was ready and I managed to mix my e.coli broth with it to make my pour plates.After a lovely big mac meal with free cheeseburger I isolated my phages using a centrifuge and a syringe containing 0.22 micron filter. I then mixed these with the soft agar. The soft agar containing the phages was placed on the top of the pour plates. This was then incubated at 37 degrees Celsius so the phages can interact with the e.coli in the nutrient agar. So to summarise the theory: The plates contain a bottom layer containing nutrient agar and e.coli where the top layer contains soft agar with the phages so hopefully the phages will interact with the e.coli overnight. I know you Biomed know this already so soz. Although I might not get the results first time, I now have confidence in doing plaque assays and feel this project could be a pretty sick one.