Starting to get set up in the lab now – another week and we might get some interesting results to post!
1. Alkaline lysis solutions made up, ready for plasmid extraction for the stability plasmid experiments for Sophie. Struggling with getting my E. coli cultures revived after they were left to die off in the fridge, but I’m confident I’ll get them back.
2. Lysis buffers set up ready for bulk NA extraction from dog testes (tomorrow’s activity) so everyone can have a go at some guaranteed successful gel electrophoresis results before getting stuck into the much more temperamental PCR experiments.
3. Plates from water from the Brayford Pool incubating – planning on getting some prelim data on total viable counts from the pool on a more or less daily basis for Ryan, to help him plan sample sizes when he gets started. Also got the MLSA plates made to start culturing E. coli for Rosie.
4. Box of random fibre samples located and tidied a little, so back on with the plan of writing a book of images for forensic fibre analysis, and revisiting some of my final year students projects to see if I can get publishable data with a few more experimental repeats.
That’s my round-up – what about yours?