Unloaded autoclave, poured 1200ml nutrient agar plates and 400ml MEA plates and set universal jars as slope jars. Learned that plates that are to be used straight away can be made a little thinner than those being made as stock to be stored.Gram stained first set of culture plates and verified as much as possible E.coli. Little more relaxed today, long lunch and a little waiting for autoclave to complete its 2hr cycle. still cant wait for tomorrow though!
Unloaded autoclave poured 1200ml nutrient agar plates and…
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Yes, just to let everyone know, we make up agar in batches of 400ml. Most places will suggest plates should contain 20ml of agar, so you would get 20 out of that batch, but I often get around 25, and sometimes I’ll push to get more, if I’m using the plates up in a day or two.