Hello,
Today myself and Mike started by discussing the gel we ran last fri-sat. We produced pretty good results, as can be seen in the image attached. We could see much clearer bands, due to increased DNA, and slightly modified settings. 
We therefore decided to start produced new plugs using the 10 different strains of Propionibacterium acnes we tried to use before. Mike re cultured these strains yesterday, therefore we left them for today to grow further, until tomorrow.
We decided to run the old plugs we produced using these 10 different strains, to confirm lack of DNA. We removed the buffer the plugs were in, added 1ml per plug of SpeI 1x wash buffer, to saturate for an hour at R.T. We then added 0.3ml SpeI buffer, 3ul BSA and 7.5ul SpeI enzyme to each plug and incubated overnight, and through tomorrow at 37C. We did have some issues with the SpeI enzyme, due to a lack of enzyme from our original batch, which was 50,000u/ml. The new SpeI enzyme was 10,000u/ml and therefore required some modified calculations, taking into account increased incubation time.
Tomorrow we will be checking the growth of the 10 different strains of Propionibacterium acnes and then beginning new plug making, if appropriate. We will also be removing the old plugs from SpeI enzyme, buffer and BSA, and washing them in 1x wash buffer before storing them till weds.