Just had a check on the agar plates…

Just had a check on the agar plates from yesterday. There is zero growth thus far on the bones that were alcohol swabbed and only minimal growth on the bones handled with gloves. This is what i expected to see and hopefully when we compare to the plates we start growing tomorrow, we will be able to say whether or not wearing gloves makes a difference.

However due to the state of the incubators and the incubation room, as well as working shoulder to shoulder with other people, i think its important to note that any results we get are only indicators and not a definitive answer, so don’t expect to just go downstairs and start fondling with one of the bodies without protection!!!!!

Glad we are in our final week, i would like to enjoy some of the summer sun before we lose it!!!

Going over the results we gathered from our…

Going over the results we gathered from our background swab of the bones and can’t help thinking how frustrating research is!!!! Why are all my bones growing different stuff on them, and why is it every time i do a repeat i get a different result!!!! Why can’t it all be as expected and work out for a nice simple conclusion????

I guess thats just the nature of research, and we can’t make the evidence fit the conclusions we want, the conclusions must fit the evidence.

Don’t get me wrong i’m still enjoying the research and my experiances this summer, its just the lazy part of me wishing it was all black and white and i could get a simple logical answer.

Right our project has finally begun We started…

Right our project has finally begun!!!!!! We started swabbing the bones as they were ie. no cleaning or handling- these swabs were placed in ringers and pipetted out onto nutrient agar and left to grow at 37 degrees for 48 hours. The result showed very little growth on the majority of plates 1-3 colonies on most or none at all!!! Except a few plates which had become overrun by a “Fuzzy mass” We are now using gram staining and subculturing to identify the colonies and the “Fuzzy mass”

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Another long update post must remember to do…

Another long update post, must remember to do this more regularly!!!

So polywipes for our project still haven’t come in, but while we wait we are using spare supplies to see how long bacteria prevails on skeletal material. Unfortunately the results were a bit screwed up, so we have had to start again. On the bright side, despite the screwy results it looked like if there was anything on the bones it was gone after 24 hours, hopefully our repeat will show the same.

Also been very busy dealing with open days and work experiance students. On wednesday i think it was (Open day) we could barely do anything because of the prosepective new students and their families buzzing in and out asking questions. Had a workshop earlier last week as well, which was fun, teaching a class about how to build a biological profile from skeletal remains; some of the kids were really enthusiastic and eager, but as always there were a few who messed around and had me going up the wall!!!!!

Had a work experiance kid in with us for one day. His name was Luke, we showed him the bones and had him attempt to age, sex and calculate stature of one of the remains, and then showed him blood spatter and got our friend Hannah (Who is working with Dr Croxton on the composition of fingerprints) to talk about her project before we handed him back to Deborah for the afternoon, hope he had a good time.

All in all a busy and frustrating week, but i keep learning new tricks and tips and am still having a good time. Hopefully come wednesday i will have definitive results on the persistance of microbes on skeletal material (Just waiting overnight while the plates incubate) and possibly have our final supplies for the main project

Hey finally round to my second of these…

Hey finally round to my second of these.

The catchup is that we have cleared our plan with Clare for the micro, and have poured a couple of Agar plates and are now waiting on results of our negative control (Blank sponge) hopefully tht will come back as expected and we can begin processing our samples next week.

Learnt how to use the stomacher with Sophie and had a good time in micro, fascinating what some of the guys are doing research on even if some of it is murdering caterpillars!!!!!!!

The fiskerton skull that Jacob is working on is now free of the massive lump of clay, and the skull is nearly clean and ready for analysis. It took 3 days to extract the clay!!!!

Sophie and i also trialed swabbing bones with ethanol to disinfect them, as reccomended by a guy at the university of Indianapolis, glad to say the bones were not damaged in any way (Dread to think what Gillian would have done if they were damaged)

Overall very good time on this research and the things in between like school visits. We had people from two of the three school groups pass out in gen lab 3, starting to think the room is cursed?!?!? I myself believe am getting very good at talking about the bones and how to analyse them, Gillian emabarrased me slightly by testing how to side bones and i failed; so will now work on that for next time.

Hopefully be living in SB209 from next week, so see you then

Right have been a little late setting this…

Right have been a little late setting this up so here goes,

My names Dan and i’m working on a UROS project alongside Sophie Webber, under the superfision of Gillian and Clare. The project is looking at whether or not wearing gloves when handling skeletal material matters. Obviously in forensic cases gloves should always be worn, but with archaeological remains there is great debate about the need to wear gloves. Hopefully by the end of the project we will be able to see whether or not there a scientifically significant transfer of microbiota from handlers to the bones if not wearing gloves. If there is a significant difference in the quantities of microbiota on skeletal material when not where wearing gloves (In comparison to wearing gloves), the next step is to see whether or not the microbiota transeferred from hands actually degrade the bones themselves.

Thats the basics behind our project. now to bring you up to speed with whats happened in the first 2 weeks.

In the first week, i met with Gillian, Clare and Sophie to discuss the project and what we would be doing. Gillian set us off familiarising ourselves with the bones of the body- learning their names, their anatomical positions, and how to sex, age and obtain stature estimations from the bones concerning the individual- this was a great start point and also really boosts my chances of passing the anthropology exam in October/November.

Following this we were then asked to identify which bones in the universities skeletal collection required cleaning, and then to clean those bones. Cleaning bones is a slow process, but oddly hypnotizing so time flies by.

So thats the important stuff thats happened over the past 2 weeks, which doesn’t sound much but the time spent just examining the bones and learning from Gillian has greatly increased my knowledge. Next week Sophie and i will be joining you in the microlab and the project can really get underway.

The two of us have been working closely with another of Gillian’s UROS students, Jacob Abbott, who has been tasked with cleaning and analysing a skull found in Fiskerton LIncolnshire, that is believed to be Iron age!!!!!! How cool is that!!!!!!!! Unfortunately he first has to work out how to remove a huge lump of clay from inside the skull without damaging it, i wish him luck with that.

So i think i have said everything that needs to be said, i’m really enjoying my project thus far, and it staves off the dull boringness of being alone in Lincoln for the summer hols. See you all next week hopefully