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Hello! I think most of you know me already but incase you don’t… I’m Rachael, (just finished) 2nd year biomed.
Steve (Wade) and I are working with/for Alan and Driton on a project involving impressively fluorescent bogeys! In slightly more scientific terms we’re investigating targeted drug delivery. Alan kindly performed the geeky part of creating the GFP-fusion (GFP-green fluorescent protein) which we transformed our tame E. coli with. This has proved very successful and although not at all required SDS-PAGE has confirmed the presence of this protein in our cells so, as Alan’s said, we’re now on to up-scaling and producing masses of bright bogeys ready for some spectroscopy and purification.
On the cell culture side of things with Driton, we received two flasks of CACO-2 intestinal epithelium cells last Thursday? (I think). These are growing well and one flask has been passaged already and the other should be ready tomorrow. It’ll take approx. 3 weeks for the cells to be ready but once they are we can start performing our ‘drug trials’ to basically see if cell uptake of the protein is successful and where it goes 🙂
I’ll try and keep you updated and might even try being a little more sciencey with it!… doubt it though….