Tag: C2110

Today may be Friday but because of the…

Deborah / / Uncategorized

Today may be Friday but because of the bank holiday monday, i am treating today like a monday!!
I am growing two batches of C2110 up to an OD of 0.35. Then i will perform cell competancy and transformation. Normally i would put the spread plates in the 37C incubator overnight but because of the bank holiday i am placing the plates in the 25C until tuesday. Hopefully there will be some growth. If all goes well Tuesday i will be working with pNC0412B, pOG04NJC, pALA1029 and pOG4003.

Today has been an almost perfect day in…

Deborah / / Uncategorized

Today has been an almost perfect day in the lab.
pOG4 and pNC0412B have grown quite nicely to an OD of 1 (0.8-1), I have performed a serial dilution on both and left the innoculated broths overnight in the shaker at 37C, the spread plates of 5 & 5.5 are in the 37C incubator. Also today i innoculated another flask of LB broth with C2110 and grew to an OD of 0.35. Then i carried out cell competancy then transformed them, using the plasmids pOG04 NJC and pNC0412B. I spread plated pOG04NJC onto an LB agar plate containing amp50 and spread plated pNC0412B onto an LB agar plated containing chl10. these were then placed in the 37C incubator overnight. Busy day tomorrow as agars, broths, velvets and NaCl need to be made-up/autoclaved, as well as tomorrows tasks. Early start i think!

Hi all Counted the t end plates from…

Deborah / / Uncategorized

Hi all,
Counted the t end plates from yesterday and recorded the results, will spend the weekend doing some scary maths. My streak plate of C2110 grew nicely so i now have a fresh sample to work with on monday. And FINALLY i have managed to get some growth from pNC0412B so i have refridgerated the plate and will be working with the chloramphenical resistant plasmid on monday, pOG4 has shown a little growth so i have placed it in the 25C incubater. Hopefully enough will have grown by monday so that i can complete the whole set of plasmids i was given to test. Am really looking forward to monday.