Most summers we have around 20 students working in the Microbiology and Molecular Biology lab, SB209, in the Science Building on the Brayford Pool campus. This is a place for those students to share their experience.
//Hey,
Yesterday i grew C2110 E. coli to an OD of 0.35. Three hours later!! I made the cells competant, then i transformed them, leaving them in the 37C shaker for 45 minutes. Once this was complete i spread plated 0.1ml onto an LB agar plate containing amp50 and left the plates to incubate overnight at 37C. Unfortunately this morning NOTHING had grown. very frustrating, so today is now monday and i’m repeating everything again!!
//Really quiet day in the lab, today was the first day of my Uros, plasmid stability experiment. Started the day by growing E.coli C2110 to an OD of 0.35, tough to guess when this happens, 0.38 was the final reading. Whilst waiting for the cells to grow i then innoculated another flask of LB broth with C2110 and placed it in the 37C shaker.The next task was to make the cells competant and then transform them, using the plasmids pOG04 NJC and pOG04 DRW. The final step was to innoculate an LB agar plate (containing Ampicillin 50) and incubate overnight at 37C.