Tag: competant cells

Groundhog day!!!!
Today is still Monday, no overnight growth again. Think C2110 is mocking me. I have repeated everything once again but i have tried to trouble shoot as to why nothing is working. Once i made thre cells competant i plated a sample onto LB agar plate and a sample onto LB agar with amp50. Then i continued on to the transformation stage, i then plated a sample onto LB agar plate and onto LB amp50 agar plate. I have also restreaked a fresh nutrient plate with C2110. Hopefully things will grow overnight, if not maybe i can work out at what stage things are going wrong.