Evening Firstly i checked on the Pseudomonas B263…

Evening,
Firstly i checked on the Pseudomonas B263 that i had made into stabs and slopes, all are showing excellent growth, another culture cleared from my bench! next Sharon and i made a few slides using lactophenol cotton blue as we still need to practice our basic techniques. Then we Gram stained a few more of the bacterial cultures on my workbench, confirming as much as we can that they are what they should be. Because a few of my bacteria are in th same family and look the same under the microscope an indole test is required to confirm their identity.I have checked that we have everything required to carry out the test, so the job for morning is now to make up the broths ready for the test.

Hi all Checked Lactobacillus plates Two do not…

Hi all,
Checked Lactobacillus plates, Two do not look like the other three, after Gram staining a sample colony from all the plates, two had Gram positve small cocci. The other three showed long rod shaped gram negative chains, ths was more like what i expected to find. To be sure, i restreaked these onto M17agar with added Lactose and incubated them overnight at 37C. In preparation for tomorrows PCR tests i made up the solutions needed, working out quantities of HCl and Tris-HCl in 200mM. Microbiology is fun but maths makes my head hurt!!! big thanks to nicola for her little equation, i now can work molar solutions out but i think more practice is definately needed