Tag: Gram stain

Hi Started the day Gram staining E coli…

Deborah / / Uncategorized

Hi,
Started the day Gram staining E.coli a strain i dont have, this is another cultue confirmed so i then made a broth which i am incubating overnight at 37C. Spent most of the day waiting for cells to grow to an OD of 1, though 0.8 – 1.5 would be within range. Nicola went through serial dilution with me and showed me how to do the first set, i then completed the following three, broth culturing -4 dilution and spread plating -5 and -5.5 dilutions onto LB agar plates and incubated them overnight at 37C. Hopefully tomorrow we will have enough growth to count colonies (100 would be nice) then we can do replica plating. Had another Mike Shaw masterclass and debate (loving lab life xx).

Right our project has finally begun We started…

Dan Firth / / Uncategorized

Right our project has finally begun!!!!!! We started swabbing the bones as they were ie. no cleaning or handling- these swabs were placed in ringers and pipetted out onto nutrient agar and left to grow at 37 degrees for 48 hours. The result showed very little growth on the majority of plates 1-3 colonies on most or none at all!!! Except a few plates which had become overrun by a “Fuzzy mass” We are now using gram staining and subculturing to identify the colonies and the “Fuzzy mass”

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Hi today luke did water filtration with water…

Deborah / / Uncategorized

Hi,
today luke did water filtration with water from the Brayford and innoculated nutrient agar plates with the filtered discs and incubated them at 30C for two days. The mannitol salt agar plates that had been spread plated with water from the Brayford showed large yellowy mucoid colonies, this was a positive for a Staphylococcus species. Using a sample from the mannitol salt plates and from the E.coli luke streaked the day before, Sharon and Luke performed Gram stains to compare the colonies. We then made up 1000ml of 50X TAE, and a 1% agarose gel for Steve. I have confirmd two more of my bacterial cultures, hopefully tomorrow i can inoculate them into broths and then into stabs and slopes.

A really successful day Yesterday i streaked 4…

Deborah / / Uncategorized

A really successful day!
Yesterday i streaked 4 Pseudomonas CFC agar plates with cultures of PS aeruginosa.Today the plates showed excellent growth and the cultures glowed florescent green. Gram staining showed that the cultures were Gram negative and rod shaped. All this was evidence that i had a pure strain of PS aeruginosa, I innoculated nutrient broth and incubated it overnight at 37C. Tomorrow i will make up two stabs and two slopes. One confirmed bacterial culture down twenty seven more to go!!! Using the Qcapture pro (microscope attached to a computor) i saved images of my Gram stained cultures to usb, hopefully i will post pictures soon.

Today I have seen some really good growth…

Deborah / / Uncategorized

Today, I have seen some really good growth on my streaked agar plates. Pseudomonas is growing nicely on CFC agar, and i am going to make up some agar to selectively grow Lactobacillus. Tomorrows task is to Gram stain the agar plates that have good single colony growth. My friend google will help me to identify the Gram stained bacterial cultures.

Today Rachael and I started to isolate single…

Deborah / / Uncategorized

Today Rachael and I started to isolate single colonies from the agar plates we incubated yeserday and streak them onto fresh nutrient agar plates. These have been placed in the 37C incubator overnight. Hopefully if there is growth, we can start to Gram stain them, further research will help us to correctly identify them and make up more stabs and slopes. We also streaked a number of yeasts onto MEA and placed them into the 25C incubator. I learned that it is better to incubate some cultures at a lower temperature for longer than at a higher temperature as this could possibly kill some cultures, especially if the higher temperature is at the top end of their tolerance range. Whilst looking at the agar plates from yesterday the P.S. aeruginosa looked cloudy and smeary, very similar to B. cereus and not the expectation of P.S. aeruginosa. We Gram stained a couple of slides using P.S. aeruginosa they were Gram positive, our research showed that it should have been Gram negative, rod shaped. Because of this we are going to streak P.S aeruginosa on Pseudimonas CFC selective agar. Hopefully tomorrow we shall see some results. Thanks to Nicola and Mike i am learning to tag,italics and google chrome.