Tag: indole test

Evening all Started out the day by performing…

Deborah / / Uncategorized

Evening all,
Started out the day by performing an indole test, confirmed Kl oxytoca i have made a broth so that on monday i will make up stabs and slopes. Once the Kovacs reagent was added to Kl oxytoca a cherry red ring immediately formed on the top of the broth, meaning that it was a positive result and that Kl oxytoca has the ability to cleave indole from the amino acid Tryptophan. Also i have brothed up Lactobacillus and incubated at 25C so that it will be ready to make up into stabs and slopes on monday. My A.baumanni stabs and slopes are incubating at 37C until tomorrow. I have restreaked a couple of plates Ent. cloace, Enterbacter spp and Serratia marascens hopefully i will be able to confirm these next week. My work bench is clearing!!!

Evening Firstly i checked on the Pseudomonas B263…

Deborah / / Uncategorized

Evening,
Firstly i checked on the Pseudomonas B263 that i had made into stabs and slopes, all are showing excellent growth, another culture cleared from my bench! next Sharon and i made a few slides using lactophenol cotton blue as we still need to practice our basic techniques. Then we Gram stained a few more of the bacterial cultures on my workbench, confirming as much as we can that they are what they should be. Because a few of my bacteria are in th same family and look the same under the microscope an indole test is required to confirm their identity.I have checked that we have everything required to carry out the test, so the job for morning is now to make up the broths ready for the test.

Hi all Interesting day started the day by…

Deborah / / Uncategorized

Hi all,
Interesting day, started the day by making 3000ml of TAE buffer. Next i ran a PCR test with a sample of my hair. When we looked at the gel under the UV some showed two bands and some showed just one band, either i’m a boy or luke is a girl. Think we may need to redo the test and look up which banding type (1 or 2) shows a male or female. Researched a few of the bacterial cultures on my bench, some are same species where an indole test is the only way to differentiate them.