Tag: Lactobacillus

Evening all Started out the day by performing…

Deborah / / Uncategorized

Evening all,
Started out the day by performing an indole test, confirmed Kl oxytoca i have made a broth so that on monday i will make up stabs and slopes. Once the Kovacs reagent was added to Kl oxytoca a cherry red ring immediately formed on the top of the broth, meaning that it was a positive result and that Kl oxytoca has the ability to cleave indole from the amino acid Tryptophan. Also i have brothed up Lactobacillus and incubated at 25C so that it will be ready to make up into stabs and slopes on monday. My A.baumanni stabs and slopes are incubating at 37C until tomorrow. I have restreaked a couple of plates Ent. cloace, Enterbacter spp and Serratia marascens hopefully i will be able to confirm these next week. My work bench is clearing!!!

Hi all Checked Lactobacillus plates Two do not…

Deborah / / Uncategorized

Hi all,
Checked Lactobacillus plates, Two do not look like the other three, after Gram staining a sample colony from all the plates, two had Gram positve small cocci. The other three showed long rod shaped gram negative chains, ths was more like what i expected to find. To be sure, i restreaked these onto M17agar with added Lactose and incubated them overnight at 37C. In preparation for tomorrows PCR tests i made up the solutions needed, working out quantities of HCl and Tris-HCl in 200mM. Microbiology is fun but maths makes my head hurt!!! big thanks to nicola for her little equation, i now can work molar solutions out but i think more practice is definately needed

Today I have seen some really good growth…

Deborah / / Uncategorized

Today, I have seen some really good growth on my streaked agar plates. Pseudomonas is growing nicely on CFC agar, and i am going to make up some agar to selectively grow Lactobacillus. Tomorrows task is to Gram stain the agar plates that have good single colony growth. My friend google will help me to identify the Gram stained bacterial cultures.