Tag: nutrient agar

Hi all another quiet day in the lab…

Deborah / / Uncategorized

Hi all,
another quiet day in the lab but very interesting. First started by collecting water from the Brayford, then Sharon spread plated, MEA, Mannitol salt, Baird parker, MLSA and nutrient agar plates. Sharons project was to count colony numbers, now that is complete, we were curious as to the type of colonies. Next i innoculated a couple of MEA slopes with the three confirmed funghi on my work bench and made a couple of MEA broths using the two funghi i have as yet not been able to culture. Saccharomyces spp and Mucor you will not defeat me!!! Nicola had a meeting which clashed with the timing of her cells being ready for competency, so she talked me through the process. Really loved it, want to learn more about anti microbial resistance.

Hi today luke did water filtration with water…

Deborah / / Uncategorized

Hi,
today luke did water filtration with water from the Brayford and innoculated nutrient agar plates with the filtered discs and incubated them at 30C for two days. The mannitol salt agar plates that had been spread plated with water from the Brayford showed large yellowy mucoid colonies, this was a positive for a Staphylococcus species. Using a sample from the mannitol salt plates and from the E.coli luke streaked the day before, Sharon and Luke performed Gram stains to compare the colonies. We then made up 1000ml of 50X TAE, and a 1% agarose gel for Steve. I have confirmd two more of my bacterial cultures, hopefully tomorrow i can inoculate them into broths and then into stabs and slopes.

Hi all Restreaked a few nutrient agar plates…

Deborah / / Uncategorized

Hi all,
Restreaked a few nutrient agar plates, hoping to find pure colonies of the target bacterial cultures that were incubating. One of the cultures didnt need to be streaked, as it was a confirmed bacteria, chromobacterium violeceum. Confirmed as it produces low, convex colonies with a dark purple/metallic sheen (violacein production). This culture, using aseptic techniques, was innoculated into nutrient broth and incubated at 30C for a coulpe of days. Then it will be made into stabs and slopes for long term storage. Researching more on selective agars to confirm more of the cultures on my lab bench. Made up more nutrient agar plates, counted colonies from Sharons Brayford pool project, cleaned lab – quite theraputic. Can’t wait to see what tomorrow brings.