Evening Firstly i checked on the Pseudomonas B263…

Evening,
Firstly i checked on the Pseudomonas B263 that i had made into stabs and slopes, all are showing excellent growth, another culture cleared from my bench! next Sharon and i made a few slides using lactophenol cotton blue as we still need to practice our basic techniques. Then we Gram stained a few more of the bacterial cultures on my workbench, confirming as much as we can that they are what they should be. Because a few of my bacteria are in th same family and look the same under the microscope an indole test is required to confirm their identity.I have checked that we have everything required to carry out the test, so the job for morning is now to make up the broths ready for the test.

A really successful day Yesterday i streaked 4…

A really successful day!
Yesterday i streaked 4 Pseudomonas CFC agar plates with cultures of PS aeruginosa.Today the plates showed excellent growth and the cultures glowed florescent green. Gram staining showed that the cultures were Gram negative and rod shaped. All this was evidence that i had a pure strain of PS aeruginosa, I innoculated nutrient broth and incubated it overnight at 37C. Tomorrow i will make up two stabs and two slopes. One confirmed bacterial culture down twenty seven more to go!!! Using the Qcapture pro (microscope attached to a computor) i saved images of my Gram stained cultures to usb, hopefully i will post pictures soon.

Today I have seen some really good growth…

Today, I have seen some really good growth on my streaked agar plates. Pseudomonas is growing nicely on CFC agar, and i am going to make up some agar to selectively grow Lactobacillus. Tomorrows task is to Gram stain the agar plates that have good single colony growth. My friend google will help me to identify the Gram stained bacterial cultures.