Most summers we have around 20 students working in the Microbiology and Molecular Biology lab, SB209, in the Science Building on the Brayford Pool campus. This is a place for those students to share their experience.
//Today i counted my T0 plates and replica plated T end plates, I have tried again with pNC0412B, pOG04 NJC and pOG04 DRW. Hopefully tomorrow there will be growth as i have used the original plasmid preps. Time to finish up with the maths and percentages as there is only 1 week left to go!!
//Hi all,
counted yesterdays plates and replica plated pOG4 onto LB and LB containing amp100, then i counted and replica plated pnc0412B onto LB and LB containing chl20, then i incubated them overnight at 37C. My next task was to complete the second serial dilution and spread plated 10-5.5 and 10-6 onto LB agar plates with no antibiotics. Next i checked on pOG04NJC and pnc0412B – NO GROWTH!!! I am sure they know its monday! Having Checked with Ross williams he thinks it could be the plasmid at fault. Tomorrow i try again.
//Hi,
Had a good couple of days, have been having trouble with growth – not yesterday each plate had 500+ colonies, think i need glasses now!! Today was an easy day too, counted yesterdays T end plates and recorded the results, then counted the plates from yesterdays serial dilution T0 plates and replica plated the ones with around 100 colonies, once again there was good growth so i replica plated ones with around 200 colonies.