Tag: TAE

Evening all x Yesterday did a PCR test…

Deborah / / Uncategorized

Evening all x
Yesterday did a PCR test with my cheek cells, mouthwash was yuck! but good news is i’m human. Today was a day of filling in COSHH and risk assessment forms, definately NOT a fun part of my day, but necessary. Confirmed M.luteus so made up stabs and slopes, Chromobacterium CV026 also confirmed, made into stabs and slopes. Made up a batch of TAE and another litre of 50x (Tris base, glacial aceitic acid, EDTA and made up to 1L with distilled water). The main thing i learned, is to not go to lunch and leave the tris to dissolve. increased pressure made it near impossible to remove the stopper, fortunately we are scientists – heating the glass whilst running cold water on the lid, hey presto, the stopper comes off.

Quiet day in the lab today A few…

Deborah / / Uncategorized

Quiet day in the lab today!
A few of the yeast cultures i had did not grow after their first innoculation onto MEA plates. I have re-streaked Mucor, Aspergillus niger and Saccromyces spp onto fresh MEA plates and incubated them at 30C. Penicillin notatum and Aspergillus parasitucus successfully grew and i have re-streaked them onto fresh MEA plates and incubated them for two days at 30C, from this i hope to gain a pure strain of the target funghi. Confirmed Chromobacteruim CV026 and have made a broth culture so that i can make up long term storage stabs and slopes. Also made up a batch of TAE with Rosie and Fran. Now to research exactly what it is and does.